单位代码10635
学号112020408002420
硕士学位论文
家蚕微孢子虫LNC2914介导的
ceRNA 网络鉴定及功能研究
论文作者：邓博远
指导教师：鲁成教授
潘敏慧教授
学科专业：特种经济动物饲养
研究方向：病原与宿主相互作用
提交论文日期：2023年04月09日
论文答辩日期：2023年05月27日
学位授予单位：西南大学
中国o 重庆
2023年5月
西南大学
二零二三届硕士学位论文
家蚕微孢子虫LNC2914介导的
ceRNA 网络鉴定及功能研究
学科专业：特种经济动物饲养
研究方向：病原与宿主相互作用
指导教师：鲁成教授
潘敏慧教授
研究生：邓博远
中国o 重庆
2023年5月
Dissertation for Master Degree of
Southwest University
Identification and function of the Nosema
bombycis LNC2914-mediated ceRNA
network
Major：The Rearing of Special-type
Economic Animals
Field：Pathogen-Host interaction
Supervisor：Prof.Cheng Lu
Prof.Min-hui Pan
Candidate：Bo-yuan Deng
Chongqing,China
May,2023
目录
目录
摘要..................................................................................................................................I
ABSTRACT .....................................................................................................................V
第一章文献综述.............................................................................................................1
1.1非编码RNA ...........................................................................................................1
1.1.1非编码RNA 概述...........................................................................................1
1.1.2非编码RNA 功能研究...................................................................................1
1.1.3非编码RNA 参与病原与宿主互作...............................................................6
1.2微孢子虫概述........................................................................................................7
1.3微孢子虫非编码RNA ...........................................................................................9
1.3.1微孢子虫基因组特征......................................................................................9
1.3.2微孢子虫非编码RNA 研究进展.................................................................11
第二章引言...................................................................................................................13
2.1研究背景与目的意义..........................................................................................13
2.2研究内容..............................................................................................................13
2.3技术路线..............................................................................................................14
第三章家蚕微孢子虫感染中肠样本全转录组测序...................................................15
3.1材料与方法..........................................................................................................15
3.1.1实验材料........................................................................................................15
3.1.2主要试剂及仪器............................................................................................15
3.1.3主要试剂配制................................................................................................16
3.1.4主要实验方法................................................................................................16
3.2结果与分析..........................................................................................................21
3.2.1家蚕微孢子虫增殖特征分析........................................................................21
3.2.2全转录组样品质量检测与分析....................................................................22
3.2.3家蚕微孢子虫milRNA 的预测和鉴定........................................................23
3.2.4家蚕微孢子虫Novel_lncRNA 的预测和鉴定.............................................25
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西南大学硕士学位论文
3.3讨论.......................................................................................................................27
第四章基于全转录组家蚕微孢子虫ceRNA 网络的构建.........................................29
4.1材料与方法...........................................................................................................29
4.1.1实验材料........................................................................................................29
4.1.2主要试剂及仪器............................................................................................29
4.1.3主要试剂配制................................................................................................29
4.1.4主要实验方法................................................................................................29
4.2结果与分析...........................................................................................................30
4.2.1家蚕微孢子虫lncRNA 顺式作用基因富集分析.........................................30
4.2.2NblncRNA-NbmilRNA-NbmRNA 调控网络分析........................................32
4.2.3NblncRNA-BmmiRNA-NbmRNA 调控网络分析........................................33
4.2.4家蚕微孢子虫lncRNA 的竞争性内源RNA 调控网络分析.......................36
4.3讨论.......................................................................................................................38
第五章家蚕微孢子虫LNC2914的全长克隆及功能研究..........................................41
5.1材料与方法...........................................................................................................41
5.1.1实验材料........................................................................................................41
5.1.2主要试剂及仪器............................................................................................41
5.1.3主要试剂配制................................................................................................43
5.1.4主要实验方法................................................................................................44
5.2结果与分析...........................................................................................................52
5.2.1家蚕微孢子虫LNC2914的序列全长克隆与分析.......................................52
5.2.2家蚕微孢子虫LNC2914的表达谱分析.......................................................53
5.2.3家蚕微孢子虫LNC2914亚细胞定位分析...................................................54
5.2.4家蚕微孢子虫LNC2914对家蚕微孢子虫增殖的影响...............................56
5.3讨论.......................................................................................................................57
第六章家蚕微孢子虫LNC2914调控家蚕miR-2808a-3p 机制研究.........................59
6.1材料与方法...........................................................................................................59
6.1.1实验材料........................................................................................................59
6.1.2主要试剂及仪器............................................................................................59
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