本实验室分离到一株溶藻细菌WS,其具有明显的溶藻效果本实验研究了ws 菌培养液不同组分的溶藻效果!不同温度加热处理对WS菌培养液溶藻效果的影响, 发现WS菌培养液的上清和菌体沉淀均具有较强的溶藻效果,且加热处理有助于提 高培养液的溶藻效果通过实验排除了WS菌培养液中溶藻活性物质是糖类!蛋白 质和核酸的可能性并通过透析实验初步判定WS菌培养液中溶藻活性物质的分子 量应该小于IKD 关键词:溶藻细菌;铜绿微囊藻;溶藻活性物质;分离篡硕士学.:i报告MASTER.5TllESIS AbstraCt Algae一lysingbacteriaWSwasisolatedandwasProvedtohavestrongalgae一lysing effect.EXPer而enisweree而edouttostudythealgae一lysingeffeetofseveral comPonentsoftheWSeulture.TheresultsindieatedthatboththesuPemateand dePositionhaveverystronglysingeffeetonMicroeystisaeruginosa.Wealsofoundout thatheatingmayhelPtoenhaneethealgae一lysingeffeetofWSculture.Itwasfoundthat thealgae一lysingactivesubstanceinAlgae一lysingbacteriaWSshouldnotbe earbohydrate!ProteinandnueleieacidthroughexPerilnents.Themoleeularweightof algae--lysingactivesubstanceshouldlowerthanIKI〕throughthedialysisexPeriment. Keywords:algae一lysingbacteria; Mieroeystisaeruginosa; algae一lysingactivesubstance; isolation 多澎硕#士学位报告MASTER.5THESIS 目录 第一部分,0.//0//.0/二/,0././二00.0./000/.,0/...././00.0.0.0二00.0../0二00.0二.., 细菌溶藻物质的初步分离/.0.00/:.0././0二/////,0././二000.//00000.00//.,0二//0: 摘要二00///////0///二/二////00//.//,.0../,/二0/00,/0二000.0//././0/0/二/00, Abstract二00二00/二/0二/.,0.,00.0/.000.00.0.0/00二0.00.0二0..0.0./00..0///0.0.//,00.11 1引言0..0/0.00////..,0二0/.0000.,/.0.1..二//.0二0.0./0./.0.,0.0,0.0.00.000.0.0.,1 1.1水华的定义,.............................................................................................1 1.2常见的水华藻类及其危害...................,..................................................1 1.3溶藻细菌及其研究现状.........................................................................2 1.4实验的目的及意义.......................-.................#4 2材料与方法.,/0.0.//0..,////00二/0二0./././//,/0,0//..00..0,0/000二/00/./00..5 2.1材料.,.,...,.,..,二,...,...........,.,....................5 2.2方法......................................卜...................................................................6 3结果与分析00/000../0././/000..0.0./二/..//00.0./.000.0/000/00..,//00//0.,//二13 3.1溶藻细菌菌体和上清的溶藻效果及分析..........................................,.13 3.2培养液经不同温度加热处理后的溶藻效果及分析..................................13 3.3Molish反应一一蔡酚反应.......................................................................16 3.4考马斯亮蓝法测定WS菌培养液上清中蛋白质的含量..........................16 3.5定磷法测定WS菌培养液上清中是否含有核酸......................................17 3.6除蛋白&核酸后WS的溶藻活性实验.......................................................18 3.7透析.............................................................................,..........................19 3.8WS溶藻活性物质的溶藻效果与时间的关系........................................19 4讨论二/.0./0/0.0/.//0/,.0./00./00//.0./,///00二/././/./0,/.0/0./000/二0./0.//21 5溶藻细菌治理水华展望../0//0..//../..//.//..0/0//0//000..0000000///../000/0022