ICS65.020.20DB14B05山西省地方标准DB 14/T1177—2016大田转基因棉花外源序列PCR检测规程2016-03-30发布2016-05-30实施山西省质量技术监督局发布DB14/T1177—2016目□□次前言..........................................................................II123456789范围.................................................................................................................................................................1规范性引用文件.............................................................................................................................................1术语和定义.....................................................................................................................................................1试剂仪器材料.................................................................................................................................................2样品DNA提取.................................................................................................................................................2PCR检测基因..................................................................................................................................................4PCR反应程序及电泳检测..............................................................................................................................4样品分析结果.................................................................................................................................................5检测档案记录.................................................................................................................................................5附录A（规范性附录）检测引物....................................................................................................................6附录B（资料性附录）转基因棉花检测鉴定结果.......................................................................................7IDB14/T1177—2016前□□言