中文摘要································································································································2 Abstract ································································································································· 9 前 言··································································································································17 第一部分 ASCs 分离、培养、鉴定和 PLLA/PCL 纳米纤维支架筛选 ·······················20 引 言····························································································································20 材料与方法 ····················································································································21 结 果····························································································································33 讨 论····························································································································42 References······················································································································· 47 第二部分 ECM 改良 PLLA/PCL 纳米纤维支架性能····················································51 引 言····························································································································51 材料与方法 ····················································································································51 结 果····························································································································61 讨 论····························································································································70 References······················································································································· 74 第三部分 方向性 PLLA/PCL 纳米纤维支架上 ASCs 成平滑肌分化及兔尿道重建··76 引 言····························································································································76 材料与方法 ····················································································································76 结 果····························································································································80 讨 论····························································································································88 References······················································································································· 92全文小结······························································································································95 综 述 脂肪干细胞在组织工程中的研究进展································································98 附录 1··································································································································111 附录 2································································································································· 113 致 谢································································································································ 1141 中英文缩略词表 英文缩写 英文全称 中文全称