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微紫青霉菌菌株GXCR的生物浸矿可行性研究报告DOC

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微紫青霉菌菌株(凡拄icilliumjanthinellum)GxCR高抗多种重金属盐(包 括铜在内),能产生多样的有机酸有效地溶解金属,具有潜在的浸矿能力" 本研究主要利用正交设计试验进行GXCR菌株生物浸矿试验,以寻找可行 而又经济的能从低品位氧化矿石中回收金属的生物浸矿技术" 浸出试验采用摇浸和堆浸方式对各种浸出过程参数进行优化"如培养 液起始pH,矿浆密度,矿石粒度,初始接种量" 培养液最适碳源都为蔗糖,最适氮源硝酸钠,最适碳源浓度为10%, 通过单因素试验得出最佳发酵培养液起始pH6,最佳发酵矿浆密度5%,最 佳初始接种量1.omL抱子液(浓度为3.0-107个/mL),最佳矿石粒度摇浸为 200目,堆浸为<200目" 在单因素试验中,20天后摇浸和堆浸铜的浸出率分别可达到79.98%和 19.29%"正交试验优化条件分别得出最佳发酵条件组合是:摇浸为培养液 起始pH6.0,矿浆密度5%,矿石粒度200目,初始接种量1.omL抱子液(浓 度为3.0x107个/mL),32e,恒温摇床120rPm下发酵20天,结果最高浸出率 可达到87.31%"堆浸为培养液起始pH6.0,矿浆密度5%,矿石粒度<200 目,初始接种量1.omL,32e恒温培养箱中培养静i培养20天"结果最高浸 出率可达27.76%" GxCR附着矿石表面生长,暗示其浸矿机理中可能存在直接作用;在浸矿过程中GXCR具有能分泌大量有机酸的能力,表明存在间接作用" 关键词:生物浸矿发酵浸出率STUDYONTHEFEASIBILITYOF BIOLEACHING丫VITH GXCR ABSTRACT 尸乞0icilliumjanthinellum,namedGXCR,wasastrainwhiehwashigh resistancetoheaVymetalsalt,ineludingeoPPer.Anditexhibitsgood Potentialingeneratingavarietyoforganieaeidseffectiveformetal solubilization.Ithasanabilityofbioleaehing.Inthisstudy,we investigatedGXCRtheabilityinbioleachingbyorthogonalexPeriments, inordertofindafeasibleandeeonomicalteehniquetomieobiallyreeover metalsfromoxidelow一gradeores# LeachingexPerimentswereearriedouttooPtimizevariousProcess Parametersintwoways:sha城ngleaehing(inProPellervessels fermentation)andheaPleaehing.SuehasinitialvalueofPHinsubstrate, slurrydensity,initialsPoreinoeulum,oresize#Carbonsourceofculture brothwassueroseandearbonsourceeoneentrationwas10%,nitrogen soureewasNaNO3.Inthesetwoway,theirsoPtimalfermentation conditionsuehasinitialPH6insubstrate,slurrydensity50/0,initialsPore 111inoeu一umlmLofsporessolution(approximately3.oxio7spores),oresize about200meshinshakingleaehingandaboutlessthan200meshinheaP leaching,werefoundaresultresPeetivelythroughsinglefaetorstest# InsinglefaetortestthemaximalsolubilizationforeoPPerwas79.98% inshakingleaehingand19#290/0inheaPleaching20dafter.Theresultby orthogonaldesignshowedthatoPtimumfermentationeoditionforGXCR shakingleaehingwereinitialPH6insubstrate,50/0slurrydensity,lmLof sporessolution(approximate一y3.oxio7spores),oresizeabout200mes一, 32oC,120runPerminuteinaeonsfanttemPeratureRoekingIncubator Shakerand20dforfermentation.ThemaximalsolubilizationforeoPPer reaehed87#310/0.InheaPleaehingwereinitialPH6insubstrate,5%slurry density,lmLorsporessolution(approximately3.oxlo7spores),oresize aboutlessthan200mesh,32oC,inaconstanttemPeratureeultureehest and20dforfermentation.Themaxima皿solubilizationforeoPPerreached 27.76%. GrowthofmyceliaalongthesurfaeeoforePartielesindieatedadireet ProeessofcoPPerbioleaching.Agreatdealoforganieacidsexeretedbythe GXCRsuggestedanindirectProeessofcoPPerbioleaehing# KEYWORDS:bioleaching;fermentation;solubilization IV目录 第一章前言............................................................................................ 1.1生物浸矿的概况.......................................................................................................1 1.1.1生物冶金与生物浸矿.............................................#1 1.1.2生物浸矿的应用领域.,..................2 1.2生物浸矿的发展概况..............................#3 1.2.1国外生物浸矿技术的历史................................................................4 1.2.2国内生物浸矿技术的历史........................................................,.........5 1.2.3用于生物浸矿的微生物种类及其浸出机制.........,............................5 1.2.4生物浸矿研究方向.........,...........,..............#8 1.3生物浸矿研究现状........#...#一9 1.4影响生物浸出的因素...................................#11 1.4.1营养因子...............................................................,..................#11 1.4.2氧.....................,..................................,.........................................12 1.4.3矿浆密度.........,.,...............................................................................#12 1.4.4矿石粒度.........................................................................................#..#..12 1.4.5培养液起始pH.......................................................#13 1.4.6初始接种量.........................................................................#13 1.5生物浸矿方式......................................................,14 1.5.1摇浸..............................................................,.....................................14 1.5.2堆浸............,............................,................................................14 1.6生物浸矿的特点.............................................,...#..#14 1.6.1优点..........,.,..................................,..,...............................巧 1.6.2缺点.........................................................................................................巧 1.7本研究工作的目的!意义............................#..#16 第二章材料与方法..................................,............................,........17 2.1材料..............................-.....................................................................,....-.172.1.1样品..................................................,.............................................17 2.1.2菌株..................................................................................................17 2.1.3培养液......................................................................................................17 2.1.4溶液与缓冲液....................................................,...........................18 2.1.5使用仪器..............................................................................................19 2.2方法.............................................................................................................20 2.2.1样品采集...................................................................,........................20 2.2.2铜矿摇浸试验..................................,.,.........................................23 2.2.3铜矿堆浸试验.........................................................................................28 2.2.4电镜观察及化学处理试验.......................................................,.......31 第三章结果与分析....................................................................-.........33 3.1矿石样品铜铁含量...................................................................................................33 3.1.1铁离子浓度标准曲线......................................................................33 3.1.2矿石样品铜铁含量..................................................................................33 3.2铜矿摇浸试验.......................................................,............................................34 3,2.1最适浸矿碳源的确定..............................................................................34 3.2.2最佳浸矿碳源浓度的确定....,...................,.................................35 3.2.3最佳浸矿发酵氮源的确定......................................................................36 3.2.4单因素试验....,...................".........................................................37 3.2.5正交试验.................................................................................................42 3.3铜矿堆浸试验..........................................................................................................44 3.3.1最适浸矿碳源的确定..............,.........................................................44 3.3.2最佳浸矿发酵氮源的确定.......,........,.........................,.........45 3.3.3单因素试验...............................................................,........................46 3.3.4正交试验............,............................,..,...................................51 3.4浸矿机理二,........,..........................................,.........................................,..,53 3.4.1直接作用............,..,.............................,.................................53 3.4.2间接作用................................,...........................................................58 3.5GXCR浸矿可行性分析....卜.............................................................................60第四章讨论与结论....................................................................................63 4,1浸矿可能机理.........#............................................................,.............................63 4.2GXCR浸矿#.......#........................................................................63